Optimization of Pre-treatment Incubation Period on Callus Induction Response in Anthers of Selected Rice Genotypes
نویسندگان
چکیده
Background: The microspores of five tropical japonica and indica rice genotypes were subjected to androgenic studies. effect growth regulators on callus induction studied improve the anther culture efficiency. Methods: cold pre-treatment panicles at 10°C done different days intervals viz., 5, 8, 10 12 days. uninucleate stage selected dusted after pre-treatment. anthers cultured in N6 basal media supplemented with casein hydrolysate (250 mg/L), proline silver nitrate (100 maltose (50 g/L) regulators. Result: 8 initiated calli most ten genotypes. taken for varied genotype from 32-55 frequency ranged 1.41 5.12%. responsive (Azucena, Palawan, Nira) their regeneration potential.
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ژورنال
عنوان ژورنال: Agricultural Science Digest – A Research Journal
سال: 2023
ISSN: ['0253-150X', '0976-0547']
DOI: https://doi.org/10.18805/ag.d-5745